Journal: The Journal of Immunology Author Choice
Article Title: Salmonella Flagellin Activates NAIP/NLRC4 and Canonical NLRP3 Inflammasomes in Human Macrophages
doi: 10.4049/jimmunol.2000382
Figure Lengend Snippet: Recombinant PrgI triggers the NAIP/NLRC4 inflammasome, whereas flagellin activates the NAIP/NLRC4 and the NLRP3 inflammasome in THP-1 cells. Parental wild-type THP-1 ( A , B , and D ), NLRC4 KO (A and B), NAIP KO ( C ), or NLRP3 KO (D) cells were transfected with PrgI or flagellin (A–D) or stimulated with LPS+ nigericin (B–D) in the presence of NLRP3 inhibitors MCC950 (B and C) or glybenclamide (B). ASC speck formation (B–D) and IL-1β (A) and IL-18 (A–D) release were determined at 4 h poststimulation. Mean with SD of at least three individual experiments (two replicates/experiment) is displayed. Statistical analysis was performed using two-way ANOVA followed by a Bonferroni posttest. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: Native ultrapure S. Typhimurium flagellin (tlrl-epstfla-5), ultrapure Bacillus subtilis flagellin (tlrl-pbsfla), the NLRP3 inhibitors MCC950 (inh-mcc), and glybenclamide (tlrl-gly) were purchased from InvivoGen.
Techniques: Recombinant, Transfection